Figure 1.

KRAS32R characterization with (A) its localization within KRAS NHE sequence in the promoter region; (B) its 1D proton NMR imino region at 37°C in 700 MHz spectrometer with several countable peaks; (C) its CD spectrum at 37°C with a positive peak around 260 nm and a negative peak around 240 nm characteristic of parallel G4 and (D) determination of the guanines implicated in KRAS 32R tetrads using ¹⁵N-filtered NMR spectra of samples containing ≈5% of ¹⁵N-enriched isotope. All experiments were performed in buffer 1× (50 mM KCl; 10 mM KPi; pH 6.66).