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. 2020 Aug 19;39(38):6113–6128. doi: 10.1038/s41388-020-01409-6

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© The Author(s) 2020, corrected publication 2022

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Fig. 2 — a, b Western blot and immunohistochemical analysis of YB1 expression in ccRCC tissues (N normal tissues, T tumor tissues). H-scores of YB1 immunohistochemical staining were presented. The values indicate protein expression levels relative to actin levels. c Western blot analysis of YB1 expression in renal normal epithelial cells (HK-2) and tumor cells (Caki-1, ACHN, 786-O). The values indicate protein expression levels relative to GAPDH levels. d, e Western blot validation of stable YB1 knockdown or overexpression in the indicated cell lines. The values indicate protein expression levels relative to GAPDH levels. f, g Scratch experimental analysis of the effect of stable YB1 knockdown or overexpression on cell migration. h, i Transwell experimental analysis of the effect of stable YB1 knockdown or overexpression on cell migration and invasion (***P < 0.001, **P < 0.01, *P < 0.05, compared with the corresponding control).