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. 2020 Sep 17;10:15267. doi: 10.1038/s41598-020-72159-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Test of GSM133-A4 in immunoblot of Listeria protein fractions and immunofluorescence microscopy. (A) Immunoblot of GSM133-A4 of protein fractions from the cytoplasm and cell wall of L. innocua DSM20649 (Linn), as well as E. coli BLR (DE3) extract used as a negative control. The blot shows that GSM133-A4 antibody binds to a target contained both in the cytoplasm and cell wall fractions of Listeria. The used protein ladder was Precision Plus Protein All Blue (Bio-Rad). (B) Fluorescence microscopy on alive L. monocytogenes 4b DSM 15675. The signal of GSM133-A4 (c and f) showed to be higher than both the negative control with a non-related antibody (a and d) and the anti-InlA positive control (b and e). The complete immunoblot image can be found as Supplementary Fig S9.