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Knockout circ_0001721 inhibited glycolysis in OS cells. HOS and U2OS cells were transfected with control, si-NC or si-circ_0001721. (A and B) QRT-PCR was used to detect the transfection efficiency. (C and D) QRT-PCR was used to detect glucose consumption in HOS and U2OS cells. (E and F) QRT-PCR was used to detect lactic acid production in HOS and U2OS cells. (G and H) QRT-PCR was used to detect the expression of HK2 in HOS and U2OS cells. *P<0.05.
