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. 2020 Sep 11;12:8287–8302. doi: 10.2147/CMAR.S244527

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© 2020 Gao et al.

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Knockdown of circ_0001721 inhibited OS cell proliferation, migration and, invasion, and promoted cell apoptosis and EMT. HOS and U2OS cells were transfected with control, si-NC or si-circ_0001721. (A and B) MTT was used to detect cell proliferation of cell lines (HOS and U2OS). (C and D) Flow cytometry was used to detect cell apoptosis. (E and F) Transwell assay was employed to explore HOS and U2OS cells migration and invasion abilities. (G and H) Western blot assay was applied to test the protein levels of E-cadherin, N-cadherin, and Vimentin in HOS and U2OS cells. *P<0.05.