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. 2020 Sep 11;12:8287–8302. doi: 10.2147/CMAR.S244527

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© 2020 Gao et al.

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Circ_0001982 directly interacted with miR-372-3p in OS cells. Starbase v3.0 predicted the putative site between circ_0001721 and miR-372-3p. (B and C) The dual-luciferase reporter assay was measured in HOS, and U2OS cells co-transfected with circ_0001721-WT or circ_0001721-MUT and miR-372-3p or miR-NC. (D and E) The enrichment of circ_0001721 or miR-372-3p was measured by RIP assay in HOS and U2OS cells incubated with Ago2 or IgG. (F and G) The expression of miR-372-3p was measured by qRT-PCR in OS tissues and HOS and U2OS cells. (H) The expression of miR-372-3p was negatively correlated with MAPK7 (R=−0.674). (I and J) The level of miR-1287-5p was determined using the qRT-PCR analysis in HOS and U2OS transfected with si-NC or si-circ_0001982. *P<0.05.