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. 2020 Sep 11;12:8287–8302. doi: 10.2147/CMAR.S244527

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© 2020 Gao et al.

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Overexpression of MAPK7 reversed the effects of miR-372-3P on OS cells. The HOS and U2OS cells were transfected with miR-NC, or miR-372-3P, miR-372-3P+vector, or miR-372-3P+pcDNA3.0-MAPK7. (A and B) Cell proliferation was assessed by MTT assay in HOS and U2OS cells. (C and D) Flow cytometry was used to detect cell apoptosis. (E and F) Transwell assay was employed to explore HOS and U2OS cells migration ability. (G and H) Transwell assay was carried out to detect HOS and U2OS cells invasion ability. (I and J) Western blot assay was applied to test the protein levels of E-cadherin, N-cadherin, and Vimentin in HOS and U2OS cells. *P<0.05.