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. 2020 Sep 4;10:472. doi: 10.3389/fcimb.2020.00472

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Copyright © 2020 Lee, Zhang, Kyman, Kask and Cope.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescent in-situ Hybridization (FISH) of the mice sinonasal tissues. Murine sinonasal tissues are hybridized with DAPI (blue) for tissue cells, and with FISH probes for P. aeruginosa (Alexa 594, pseudo-colored in yellow), S. aureus (Alexa 660, pseudo-colored in red) or genus Malassezia (Alexa 488, pseudo-colored in green). Hybridized specimens were observed using a confocal laser scanning microscope (CLSM) for 3 dimensional analysis. (A) Control, (B) M. sympodialis infected mouse, (C), M. sympodialis and P. aeruginosa infected mouse, and (D) M. sympodialis and S. aureus infected mouse.