Table 3.

Plate safety test results for inactivation of EBOV/Mak by the 0.5% and 1% NaOCl concentrations.

Test condition (contact time)	0.5% NaOCl			1% NaOCl
	Trial 1	Trial 2	Trial 3	Trial 1	Trial 2	Trial 3
Negative control	−, −, −	−, −, −	−, −, −	−, −, −	−, −, −	−, −, −
N + NaOCl	−, −, −	−, −, −	−, −, −	−, −, −	−, −, −	−, −, −
Positive control	+, +, +	+, +, +	+, +, +	+, +, +	+, +, +	+, +, +
NaOCl (1 min)	+, +, +	+, +, +	+, +, +	+, +, +	+, +, +	+, +, +
NaOCl (2.5 min)	+, −, +	−, +, −	+, +, +	+, +, +	−, +, +	+, +, +
NaOCl (5 min)	−, −, −	−, −, −	−, −, −	−, −, −	−, −, −	−, −, −
NaOCl (7.5 min)	−, −, −	−, −, −	−, −, −	−, −, −	−, −, −	−, −, −
NaOCl (10 min)	−, −, −	−, −, −	−, −, −	−, −, −	−, −, −	−, −, −

The Vero E6 cultures were inoculated with undiluted neutralized samples and passaged twice. +, viral CPE observed; −, viral CPE not observed, or no cytotoxicity observed for N + NaOCl (neutralizer + NaOCl cytotoxicity control) (modified from Cook et al.¹¹).