Fig. 1. TMZ-resistant GBM cell lines express a low level of DAB2IP.

a TMZ log–dose–response analysis (IC₅₀) in four human glioblastoma cell lines. Cells were treated with increment doses of TMZ as indicated for 48h, and IC₅₀ was determined with cytotoxicity results assessed by MTT assay. Percent survival curves after TMZ treatment was constructed by plotting the cell survival whose values indicate mean ± SD (n = 5). Error bars indicate standard deviation. b The mRNA expression of DAB2IP analyzed by real-time PCR in four human glioblastoma cell lines. Black and white bars indicate DAB2IP-high and DAB2IP-low cell lines, respectively. Means ± SD; n = 3; Student’s two-tailed t-test, **p < 0.01. c Representative images of western blot against DAB2IP and β-actin.