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. 2020 Sep 17;10:15316. doi: 10.1038/s41598-020-72136-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of STAT3 decoy ODN transfection on lung injury in mice following CLP-induced sepsis. Lung tissues were harvested from sham-operated and CLP-induced septic mice 18 h after surgery. (A) Lung tissue sections stained with hematoxylin and eosin. (B) Lung tissue sections were stained with antibody against MPO followed by peroxidase staining. MPO-positive cells were stained brown and indicated by red arrowheads. Scale bar, 100 μm. (C) Semiquantitative analysis of lung tissues by lung injury, which was assessed by scoring from 0 to 4 as described in Methods (n = 6). (D) Quantitation of MPO-positive cell counts in lung tissues. The average of MPO-positive cell number in five fields per sample was calculated (n = 6). ***P < 0.001 compared with the respective control (18 h after sham operation). ^###P < 0.01 compared with CLP alone.