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. 2020 Sep 4;11:580233. doi: 10.3389/fmicb.2020.580233

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Copyright © 2020 Li, Zhang, Zhang, Li, Fan, Zhu, Fan, Li, Chen, Yi, Ding, Chen and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Classical swine fever virus (CSFV) infection downregulates SERINC5 expression in tissues of CSFV-infected pigs. Tissue samples (heart, liver, spleen, lung, kidney, brain, inguinal lymph nodes, mesenteric lymph nodes, thymus, and tonsil) were collected after the pigs were euthanized at 7 dpi. Protein (A) and transcription (B) of SERINC5 in different tissues were analyzed by western blotting and qRT-PCR, respectively. The relative levels of the targeted proteins were analyzed with ImageJ software, and the ratios were calculated relative to the GAPDH control. Error bars represent the mean ± SD; n = 3; ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001; ^NSP > 0.05.