Fig. 7.

Pharmacodynamic (PD) analysis and antitumor activity of ARD-61 in the MDA-MB-453 xenograft tumor model. (A) Male SCID mice bearing MDA-MB-453 tumors were treated with a single dose of ARD-61 at 25 mg/kg by intraperitoneal injection. Mice were sacrificed and tumor tissues were collected at indicated time points for PD analysis. Data are mean ± SEM (n = 3). P values were calculated using Student’s t test. (B) Male SCID mice bearing MDA-MB-53 tumors were treated with a single dose of ARD-61 at 25 mg/kg by intraperitoneal injection. Mice were sacrificed and tumor tissues were collected at indicated time points. Total RNAs were isolated from tumor lysates for qPCR. Data are mean ± SEM (n = 3). (C, D) Male SCID mice bearing the MDA-MB-453 tumors were treated with different drugs at indicated dose-schedules. Data are mean ± SEM (n = 8).