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. 2020 Sep 4;11:555794. doi: 10.3389/fpls.2020.555794

Figure 9.

Figure 9

GmUBC9 interacts with GmHUB2/AtHUB2. (A) Interaction between GmUBC9 and GmHUB2/AtHUB2 detected by yeast two-hybrid (Y2H) assays and a quantitative assay for β-galactosidase activity. The presented values are the averages of four independent assays with standard deviations. pGADT7 (AD) and pGBKT7 (BD) were used as negative controls. AD, GAL4 activation domain. BD, GAL4 DNA-binding domain. (B) Confirmation of the interaction of GmUBC9 and GmHUB2/AtHUB2 by BiFC in Arabidopsis protoplasts. DAPI was used to stain nuclei. The white arrow indicates the nucleus. Scale bars, 10 μm.