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. 2020 Sep 4;11:2071. doi: 10.3389/fimmu.2020.02071

TABLE 1.

Phenotype of immunosuppressive, antigen-experienced Treg.

Pre-CD4+ enrichment

Marker Percent within Treg cells (CD4+CD25+FoxP3+)
Eomes+ 0.5
Helios+ 70.0
CD127+ 15.0
LAG3+ 1.0

Post-CD4+ enrichment

Eomes+ 0.0
Helios+ 97.0
CD127+ 0.8
LAG3+ 0.3

Flow cytometric characterization of CD4+CD25+FoxP3+ Treg cells obtained from dTg HEL/TCR mice, pre- and post-magnetic bead CD4+ enrichment. Representative data are provided.