Fig. 7.

Immunohistochemical images demonstrating some components of the mitochondrial lactate oxidation complex (mLOC) in L6 cells. The mLOC contains the inner mitochondrial membrane cytochrome oxidase (COX), the lactate-pyruvate transporter (MCT1), lactate dehydrogenase (LDH) and the MCT anchoring protein, CD147 (Basigin). MCT1 was detected at both sarcolemmal and intracellular domains (A-1). Mitochondrial reticulum (MR), identified by MitoTracker, was extensively elaborated in L6 cells (A-2). The merged images of MCT1 (green, A-1) and MR (red, A-2) showed intense yellow, indicating co-localization of MCT1 and components of the MR, particularly at perinuclear cell domains (A-3). (B) LDH (B-1) and COX (B-2) are imaged. Superposition of signals for LDH (red, B-1) and COX (green, B-2) shows co-localization of LDH in the MR (yellow) of muscle cells (B-3). Depth of field approximately 1 μm, scale bar = 10 μm. Similar data have been obtained on rat plantaris leg muscles in vivo. From Ref. 75 with permission.