TABLE 1.

Clinical parameters of dengue patients and pattern of different microRNAs detected in their plasma

Patient samples used for discovery
	DI	DWS		DS
No. of plasma samples	9	14		16
Age in years (mean ± SD)	24 ± 8	18 ± 6		20 ± 5
Platelet count (mean ± SD) × 103	104 ± 41	29 ± 5		15 ± 3
Duration of fever (days)	1–3	3–5		3–6
Serotype (DENV-1/2/3)	2/5/2	5/8/1		2/12/2
Patient samples used for validation
	OFI	DI		DS
No. of plasma samples	7	14		12
Age in years (mean ± SD)	28 ± 06	23 ± 09		20 ± 07
Duration of fever (days) at the time of recruitment	1–4	2–5		3–6
Platelet count (mean ± SD) × 103	95 ± 30	110 ± 55		20 ± 4
Serotype (DENV-1/2/3)	0/0/0	3/10/1		2/10/0
Follow-up patients
	Age in years (mean ± SD)	Platelet count (mean ± SD) × 103 Day 0		Platelet count (mean ± SD) × 103 Day 5
Group-1 (n = 4)	22 ± 8	116 ± 43.8		57.6 ± 13.9
Group-2 (n = 5)	25 ± 10	37.5 ± 24.6		128 ± 25.8
miRNA RNA-seq analysis
	DI	DWS		DS
No. of miRNAs detected	603	668		318
Range of miRNAs detected	138–331	151–466		68–214
Total no. of detectable miRNAs in at least two-thirds of samples	217	199		89
Differentially expressed miRNAs (DEMis)
	DI vs DWS		DI vs DS
No. of common expressed miRNAs	177		89
No. of DEMis (lfc >0.5, P value <0.05)a	74		38
Upregulated	40		21
Downregulated	34		17

^alfc, log fold change.