TABLE 1.
Biophysical and pharmacological properties of mutant Kv7.3 channels.
| n | V1/2 (mV) | k (mV/e-fold) | Current density at + 20 mV (pA/pF) | Blockade by 3 mM TEA (%) | 100 μM BHB (Δ V, mV) | |
| CHO | 10 | – | – | 0.5 ± 0.05 | – | – |
| Kv7.3 | 26 | −37.2 ± 1.1* | 7.2 ± 0.4* | 12.6 ± 1.1* | 8.0 ± 2.1* | – |
| Kv7.3 M240R | 8 | – | – | 0.5 ± 0.2 | – | – |
| Kv7.2 | 11 | −26.8 ± 1.3 | 13.2 ± 0.9 | 42.1 ± 2.5 | 85.1 ± 3.1* | – |
| Kv7.2 + Kv7.3 | 18 | −27.7 ± 1.4 | 11.2 ± 0.5 | 126.4 ± 17.9 | 50.1 ± 4.3 | −8.1 ± 1.1 |
| Kv7.2 + Kv7.3 M240R | 7 | 18.1 ± 4.9* | 22.6 ± 0.8* | 70.2 ± 10.4* | 45.2 ± 5.1 | – |
| Kv7.2 + Kv7.3 + Kv7.3 M240R | 18 | −15.6 ± 1.7* | 15.5 ± 0.7* | 115.0 ± 15.2 | 48.5 ± 5.2 | −8.7 ± 1.1 |
TEA, tetraethylammonium; CHO, Chinese hamster ovary. ∗p < 0.05 vs. Kv7.2 + Kv7.3.