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. 2020 Sep 4;8:773. doi: 10.3389/fbioe.2020.00773

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Copyright © 2020 Balion, Sipailaite, Stasyte, Vailionyte, Mazetyte-Godiene, Seskeviciute, Bernotiene, Phopase and Jekabsone.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NMR spectra of CLP, CLP-RGD, CLP-IKVAV, 8-arm-PEG (40kDa) and PEG-CLP derivatives. Blue arrows mark arginine at δ 3.10 ppm and serine peaks at δ 3.90 ppm of CLP-RGD peptide. Green arrows show valine at δ 0.75 ppm, isoleucine at δ 0.82 ppm, and alanine peaks at δ 1.22 ppm of CLP-IKVAV peptide. The maleimide peaks at δ 6.5–7.0 ppm disappeared after conjugation with CLPs peptides.