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. 2020 Sep 4;8:773. doi: 10.3389/fbioe.2020.00773

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Copyright © 2020 Balion, Sipailaite, Stasyte, Vailionyte, Mazetyte-Godiene, Seskeviciute, Bernotiene, Phopase and Jekabsone.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Size distribution of individual focal contacts (A), projected cell area, cell shape index, and number of focal contacts (B) of HROG36, C6, and A375 cells on PEG-CLP, PEG-CLP-RGD, and PEG-CLP-IKVAV hydrogels. The cells were immunostained for F-actin (red signal) and vinculin (green). Nuclei were visualized blue by DAPI. Size distribution of focal contacts of three cells on each hydrogel was plotted together with approximate distribution curve. Projected cell area and perimeter for cell shape index calculation were measured by ImageJ software. Focal adhesion number per cell was calculated according to the number of vinculin-actin colocalization points. The data are presented as AVG ± SDEV of 5–7 cells from three of each hydrogel sample. *Indicates statistically significant difference compared with the samples of the same cell type grown on PEG-CLP, ∧ – compared with samples on PEG-CLP-RGD. ANOVA with post hoc LSD test, p < 0.05.