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The expression of a HAM1 translational reporter in the SAM. (A–F) Confocal imaging of a pHAM1::YPET-HAM1 translational reporter in a SAM of a ham123 triple mutant, from the orthogonal view (A, D), transverse optical section view in L1 (B, E), and corpus (C, F). (A–C) merged channels from YFP (green) and PI (propidium iodide, purple). (D–F) merged channels from the quantified YFP (quantitatively indicated by color) and PI. Scale bar: 20 µm. Color bar: Fire quantification.
