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The expression of a HAM2 translational reporter in the SAM. (A–F) Confocal imaging of a pHAM2::YPET-HAM2 translational reporter in a SAM of a ham123 triple mutant, from the orthogonal view (A, D), transverse optical section view in L1 (B, E), and corpus (C, F). (A–C): merged channels from YFP (green) and PI (propidium iodide, purple). (D–F): merged channels from the quantified YFP (quantitatively indicated by color) and PI. Scale bar: 20 µm. Color bar: Fire quantification.
