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. 2020 Sep 4;11:541968. doi: 10.3389/fpls.2020.541968

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Copyright © 2020 Han, Geng, Guo, Yan, Meyerowitz, Liu and Zhou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression and function of pHAM2::YPET-HAM3 in the SAM. (A–F) Confocal imaging of a pHAM2::YPET-HAM3 translational reporter in a SAM of ham123 from orthogonal view (A, D), transverse view in L1 (B, E), and corpus (C, F). (A–C): merged channels from YFP (green) and PI (purple). (D–F): merged channels from the quantified YFP (quantitatively indicated by color) and PI. Color bar: Fire quantification. Scale bar (A–F): 20 µm. (G–I) RNA in situ hybridization of CLV3 in the SAMs of wild type (Ler) (G), ham123 (H), and pHAM2::YPET-HAM3 in ham123 (I) at the same developmental stage (27 DAG). Scale bar (G–I): 50 µm. At least three biological replicates were performed for each genotype with similar results.