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. 2020 Sep 4;11:541968. doi: 10.3389/fpls.2020.541968

Figure 7.

Figure 7

Roles of different HAM genes in control of CLV3 patterning in de novo stem cell niches and in branch development. (A–E) RNA in situ hybridization of CLV3 in the initiating stem cell niches of Ler wild type (A), ham123 (B), pHAM1::YPET-HAM1 in ham123 (C), pHAM2::YPET-HAM2 in ham123 (D), and pHAM3::YPET-HAM3 in ham123 (E). Scale bar: 50 µm. Arrows indicate the CLV3 expressing cells. (F–J) Images of branches initiated from base of the cauline leaves in different genotypes, which are grown in the same condition at the same age. Arrows indicate the branches initiated normally from the base of the cauline leaves of Ler wild type (F), pHAM1::YPET-HAM1 in ham123 (H) and pHAM2::YPET-HAM2 in ham123 (I), and they indicate the absence of branches initiated from the base of the cauline leaves in ham123 (G) and pHAM3::YPET-HAM3 in ham123 (J). Scale bar: 0.5cm. At least three biological replicates were performed for each genotype with similar results.