Figure 3.

Mettl14-deficient T_reg cells are unable to suppress naïve T cell–induced colitis in Rag1^–/– adoptive transfer T cell model of colitis. WT naïve T cells, Mettl14^–/– naïve T cells, WT naïve T cells + WT T_reg cells, or WT naïve T cells + Mettl14^–/– T_reg cells were transferred to Rag1^–/– mice. (A) Weight change. ∗∗P < .01 comparing mice that received WT naïve T cells + WT T_reg cells vs WT naïve T cells + Mettl14^–/– T_reg cells. (B) Colon weight-to-length ratio. (C) Hematoxylin and eosin stain of colon. (D) Relative expression of Th1 cytokine IFNγ/TNFα and Th17 cytokine IL-17a/IL-17c. Log₂ scale was used on the y-axis. (E) Flow cytometry analysis of percent of T_reg cell in mesenteric lymph nodes. WT indicates wild-type littermate control. KO indicates CD4-Cre^+/TgMettl14^FL/FL conditional knockout. Data are represented as mean ± SD; n = 4–5 mice per group. Data representative of 2 independent experiments with similar results.