Figure 6.

Mettl14 deficiency causes impaired induction of naïve T cells into iT_reg cells. (A) Induction efficiency of iT_reg cells from naïve T cells as evidenced by Foxp3 expression. (B) Representative flow cytometry image showing percent of Foxp3⁺ iT_reg cells after induction from naïve T cells. The cells were gated from a parent population of CD4⁺ cells. WT indicates wild-type littermate control. KO indicates CD4-Cre^+/TgMettl14^FL/FL conditional knockout. Data are represented as mean ± SD; n = 12 per group. Combined data from 3 independent experiments shown. (C) m⁶A profiling of iT_reg cells compared with naïve T cells. Black color indicates nonsignificant; blue color indicates P < .05 and absolute Log₂ fold change <1; red color indicates P < .05 and absolute Log₂ fold change >1. (D) Significant pathways represented by genes with differential m⁶A levels. The top 5 most significant pathways were shown.