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HPLC chromatograms of the lpsB knockout strain of M. brunneum (lpsB ko) compared to wild-type M. brunneum and LA-accumulating strain of N. fumigata. Fluorescence was detected at 410 nm after excitation at 310 nm (A) and at 372 nm after excitation at 272 nm (B). Peaks corresponding to characterized ergot alkaloids are indicated: LA/isoLA, lysergic acid/isolysergic acid; LAH, lysergic acid α-hydroxyethylamide.
