FIG 6.

HPLC chromatograms of M. brunneum mutant with novel alkaloid compared to those of parental strains from which it was derived: wild-type M. brunneum and easA knockout (easA ko) of M. brunneum. Fluorescence was detected at 410 nm after excitation at 310 nm (A) and at 372 nm after excitation at 272 nm (B). The wild-type (black line) peaks eluting just later than dihydro-LAH correspond to the most abundant LAH isomers (marked in panel A) fluorescing under suboptimal excitation and emission wavelengths. A DHLA standard was also included. LAH, lysergic acid α-hydroxyethylamide; DHLA, dihydrolysergic acid; dihydro-LAH, provisional dihydrolysergic acid α-hydroxyethylamide.