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LC-MS analyses of M. brunneum mutant with novel alkaloid compared to wild-type M. brunneum. (A to D) Extracted ion chromatograms of wild-type M. brunneum analyzed at indicated m/z values (A, C) compared to the easA knockout strain expressing the dihydro expression construct (B, D). (E, F) Fragmentation spectra obtained from parent ions shown in panels A and D. Instrument resolution accurate to 0.1 Da.
