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. 2020 Jun 8;27(10):2660–2668. doi: 10.1016/j.sjbs.2020.06.002

Table 1.

MIC values for blaTEM producing bacteria isolated from Kashmir Valley, India.

Isolates MIC value
CAZ (μg/ml) CTX (µg/ml) CIP (µg/ml) C (µg/ml) TR (µg/ml) AMP (µg/ml) CL (µg/ml) AgNO3 (mg/l) Na3ASO4 (mg/l) HgCl2 (mg/l)
AN9 <2 <2 <2 <2 <2 8 <2 8 64 16
AN5 <2 <2 <2 <2 16 <2 <2 8 32 2
BS4 <2 <2 <2 <2 <2 64 <2 8 128 16
WR7 128 256 <2 8 256 8 8 16 16 8
AN10 256 256 <2 4 >256 256 8 4 64 64
MDY3 >256 256 256 16 32 >256 16 8 256 16
AN9 32 <2 <2 4 <2 32 >256 8 256 8
WG4 256 32 <2 <2 <2 16 256 2 128 2
WG7 256 64 <2 8 <2 <2 >256 2 256 16
JN2 >256 256 32 16 256 8 4 16 128 16
WR1 8 128 4 8 16 4 >256 8 64 8

The antibiotics used for Minimum Inhibitory Concentration (MIC) determination were ceftazidime (CAZ), cefotaxime (CTX), ciprofloxacin (CIP), chloramphenicol (C), trimethoprim (TR), ampicillin (AMP) and colistin (CL) for heavy metals silver nitrate (AgNO3), mercuric chloride (HgCl2) and sodium arsenate (Na3AsO4) was used. Reference values as per Clinical laboratory Standard Institute (CLSI) guidelines for MIC determination of resistance, intermediate and sensitive phenotype are mentioned CAZ: ≥16, 8, ≤4, CTX: ≥4, 2, ≤1, CIP: ≥4, 2, ≤1, C: ≥32, 18, ≤8, TR: ≥16, ≥8, ≤8, AMP: ≥32, 16, ≤8, CL: ≥2, 2, ≤1. Reference values for the permissible limits of heavy metals in water were adapted from the available literature for Arsenic (As): 0.02 mg/L, Mercury (Hg): 0.01 mg/L Adopted from Singh, et al. 2011 (Indian Journal of Pharmacology) and for Silver (Ag): 0.1 mg/L Adopted from Alan B. G. Lansdown 2010 (Advances in Pharmacological Sciences).