Table 1.
Isolates | MIC value |
|||||||||
---|---|---|---|---|---|---|---|---|---|---|
CAZ (μg/ml) | CTX (µg/ml) | CIP (µg/ml) | C (µg/ml) | TR (µg/ml) | AMP (µg/ml) | CL (µg/ml) | AgNO3 (mg/l) | Na3ASO4 (mg/l) | HgCl2 (mg/l) | |
AN9 | <2 | <2 | <2 | <2 | <2 | 8 | <2 | 8 | 64 | 16 |
AN5 | <2 | <2 | <2 | <2 | 16 | <2 | <2 | 8 | 32 | 2 |
BS4 | <2 | <2 | <2 | <2 | <2 | 64 | <2 | 8 | 128 | 16 |
WR7 | 128 | 256 | <2 | 8 | 256 | 8 | 8 | 16 | 16 | 8 |
AN10 | 256 | 256 | <2 | 4 | >256 | 256 | 8 | 4 | 64 | 64 |
MDY3 | >256 | 256 | 256 | 16 | 32 | >256 | 16 | 8 | 256 | 16 |
AN9 | 32 | <2 | <2 | 4 | <2 | 32 | >256 | 8 | 256 | 8 |
WG4 | 256 | 32 | <2 | <2 | <2 | 16 | 256 | 2 | 128 | 2 |
WG7 | 256 | 64 | <2 | 8 | <2 | <2 | >256 | 2 | 256 | 16 |
JN2 | >256 | 256 | 32 | 16 | 256 | 8 | 4 | 16 | 128 | 16 |
WR1 | 8 | 128 | 4 | 8 | 16 | 4 | >256 | 8 | 64 | 8 |
The antibiotics used for Minimum Inhibitory Concentration (MIC) determination were ceftazidime (CAZ), cefotaxime (CTX), ciprofloxacin (CIP), chloramphenicol (C), trimethoprim (TR), ampicillin (AMP) and colistin (CL) for heavy metals silver nitrate (AgNO3), mercuric chloride (HgCl2) and sodium arsenate (Na3AsO4) was used. Reference values as per Clinical laboratory Standard Institute (CLSI) guidelines for MIC determination of resistance, intermediate and sensitive phenotype are mentioned CAZ: ≥16, 8, ≤4, CTX: ≥4, 2, ≤1, CIP: ≥4, 2, ≤1, C: ≥32, 18, ≤8, TR: ≥16, ≥8, ≤8, AMP: ≥32, 16, ≤8, CL: ≥2, 2, ≤1. Reference values for the permissible limits of heavy metals in water were adapted from the available literature for Arsenic (As): 0.02 mg/L, Mercury (Hg): 0.01 mg/L Adopted from Singh, et al. 2011 (Indian Journal of Pharmacology) and for Silver (Ag): 0.1 mg/L Adopted from Alan B. G. Lansdown 2010 (Advances in Pharmacological Sciences).