Table 3.
MIC values for blaTEM producing transconjugants isolated from Kashmir valley, India.
| Isolates | MIC |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| CAZ (μg/ml) | CTX (µg/ml) | CIP (µg/ml) | C (µg/ml) | TR (µg/ml) | AMP (µg/ml) | CL (µg/ml) | AgNO3 (mg/l) | Na3ASO4 (mg/l) | HgCl2 (mg/l) | |
| E. coli AN10 | 256 | 256 | <2 | 4 | >256 | 256 | 8 | 4 | 64 | 64 |
| AN10/Transconjugant (J53AZR) | 256 | 32 | <2 | 4 | 128 | 16 | 2 | 2 | 64 | 64 |
| Acinetobacter sp.WR7 | 128 | 256 | <2 | 8 | 256 | 8 | 8 | 16 | 16 | 8 |
| WR7/Transconjugant (J53AZR) | 128 | 256 | <2 | 2 | 64 | 8 | 4 | 8 | 8 | 2 |
For the result analysis Reference values of CLSI guidelines were used for MIC determination of resistance, intermediate and sensitive phenotype the values are mentioned as CAZ: ≥16, 8, ≤4, CTX: ≥4, 2, ≤1, CIP: ≥4, 2, ≤1, C: ≥32, 18, ≤8, TR: ≥16, ≥8, ≤8, AMP: ≥32, 16, ≤8, CL: ≥2, 2, ≤1. Reference values for the permissible limits of heavy metals in water were adapted from the available literature for Arsenic (As): 0.02 mg/L,Mercury (Hg): 0.01 mg/L Adopted from Singh, et al. 2011 (Indian Journal of Pharmacology) and for Silver (Ag): 0.1 mg/L Adopted from Alan B. G. Lansdown 2010 (Advances in Pharmacological Sciences)