Table 4.
Gene | Primer name | Primer sequence | Product size (bp) | References/Accession No. |
---|---|---|---|---|
16S rRNA | RR-F RR-R |
5′- GGCGGACGGGTGAGTAATG −3′ 5′- CGATTACTAGCGATTCCGACTTC −3′ |
1250 | This study |
blaTEM | TEM-F TEM-R |
5′-ATGAGTATTMAACATTTYCGTGTCGCC-3′ 5′-TTACCAATGCTTAATCAGTGAGGCACCTATC-3′ |
861 | This study |
AmpC | AMP-F AMP-R |
5′-GATCGTTCTGCCGCTGTG-3′ 5′-GGGCAGCAAATGTGGAGCAA-3 |
271 | Corvec et al. (2007) |
qnrA | QA-F QA-R |
5′-AGAGGATTTCTCACGCCAGG-3′ 5′-TGCCAGGCACAGATCTTGAC-3′ |
580 | Cattoir et al. (2007) |
qnrB | QB-F QB-R |
5′-GATCGTGAAAGCCAGAAAGG-3′ 5′-ATGAGCAACGATGCCTGGTA-3′ |
476 | Kim et al. (2009) |
qnrS | QS-F QS-R |
5′-GCAAGTTCATTGAACAGGGT-3′ 5′-TCTAAACCGTCGAGTTCGGCG-3′ |
428 | Cattoir et al. (2007) |
merB | MB-F MB-R |
5′-ATGAAGCTCGCCCCATATATT-3′ 5′-TCACGGTGTCCTAGATGACAT |
640 | This study |
merP | MP-F MP-R |
5′-ATGAAGAAACTGTTTGCCTCC-3′ 5′-TCACTGCTTGACGCTGGACG-3′ |
272 | This study |
merT | MT-F MT-R |
5′-TTAATAGAAAAATGGAACGAC-3′ 5′-ATGTCTGAACCACAAAACGGG-3′ |
355 | This study |
silE | SE-F SE-R |
5′-GTACTCCCCCGGACATCACTAATT −3′ 5′-GGCCAGACTGACCGTTATT-3′ |
410 | Percival et al. (2008) |
silP | SP-F SP-R |
5′-GGCGATAAGCTCCGCATCAGA-3′ 5′-TCCACT TTT TCAAGACGCTCA-3′ |
524 | Kremera and Hoffmann (2012) |
silS | SS-F SS-R |
5′-GGAGATCCCGGATGCATAGCAA-3′ 5′-GTTTGCTGCATGACAGGCTAA AGACATC-3′ |
1500 | Percival et al. (2008) |
arsC | AR-F AR-R |
5′-GTAATACGCTGGAGATGATCCG-3′ 5′-TTTTCCTGCTTCATCAACGAC-3′ |
409 | Sunita et al. (2012) |
CS | 5′CS 3′CS |
5′-GGCATCCAAGCAGCAAG-3′ 5′-AAGCAGACTTGACCTGA-3′ |
530 | M73819 |
IntI |
IntI- F IntI -R |
5′-CTACCTCTCACTAGTGAGGGGCGG-3′ 5′-GGGCAGCAGCGAAGTCGAGGC-3′ |
845 | U12338 |
SulI | SulI-F SulI-R |
5′-ATGGTGACGGTGTTCGGCAT-3′ 5′CTAGGCATGATCTAACCCTC-3′ |
800 | Galimand et al. (2003) |
ISecp1 | IS-F IS-R |
5′-TAAAAAACACAGGTGGAATTTAG-3′ 5′-CCAGGAACCACGGAGC-3′ |
1000 | This study |
TN3 | Tn3 P-1 Tn3 P-2 Tn3 P-3 |
5′-AACTGATCTTCCTGACCGTC- 3′ 5′-TATGACCGATACGGCAGGTG- 3′ 5′-TCAGCAATGAACGGACCAGC- 3′ |
752 | Heffron et al. (1979) |
TN21 | Tn21 P-1 Tn21 P-2 Tn21 P-3 |
5′-AGAAAGTTCGTCCTGGGCTG- 3′ 5′-TACTGCCGCGCATCAAGATC-3′ 5′-GGCCAAGGACAAGAACCTGT-3′ |
1000 | Kim et al. (2009), Brown et al. (1985) |