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. 2020 Jun 8;27(10):2660–2668. doi: 10.1016/j.sjbs.2020.06.002

Table 4.

Primers used for amplification of different genes.

Gene Primer name Primer sequence Product size (bp) References/Accession No.
16S rRNA RR-F
RR-R
5′- GGCGGACGGGTGAGTAATG −3′
5′- CGATTACTAGCGATTCCGACTTC −3′
1250 This study
blaTEM TEM-F
TEM-R
5′-ATGAGTATTMAACATTTYCGTGTCGCC-3′
5′-TTACCAATGCTTAATCAGTGAGGCACCTATC-3′
861 This study
AmpC AMP-F
AMP-R
5′-GATCGTTCTGCCGCTGTG-3′
5′-GGGCAGCAAATGTGGAGCAA-3
271 Corvec et al. (2007)
qnrA QA-F
QA-R
5′-AGAGGATTTCTCACGCCAGG-3′
5′-TGCCAGGCACAGATCTTGAC-3′
580 Cattoir et al. (2007)
qnrB QB-F
QB-R
5′-GATCGTGAAAGCCAGAAAGG-3′
5′-ATGAGCAACGATGCCTGGTA-3′
476 Kim et al. (2009)
qnrS QS-F
QS-R
5′-GCAAGTTCATTGAACAGGGT-3′
5′-TCTAAACCGTCGAGTTCGGCG-3′
428 Cattoir et al. (2007)
merB MB-F
MB-R
5′-ATGAAGCTCGCCCCATATATT-3′
5′-TCACGGTGTCCTAGATGACAT
640 This study
merP MP-F
MP-R
5′-ATGAAGAAACTGTTTGCCTCC-3′
5′-TCACTGCTTGACGCTGGACG-3′
272 This study
merT MT-F
MT-R
5′-TTAATAGAAAAATGGAACGAC-3′
5′-ATGTCTGAACCACAAAACGGG-3′
355 This study
silE SE-F
SE-R
5′-GTACTCCCCCGGACATCACTAATT −3′
5′-GGCCAGACTGACCGTTATT-3′
410 Percival et al. (2008)
silP SP-F
SP-R
5′-GGCGATAAGCTCCGCATCAGA-3′
5′-TCCACT TTT TCAAGACGCTCA-3′
524 Kremera and Hoffmann (2012)
silS SS-F
SS-R
5′-GGAGATCCCGGATGCATAGCAA-3′
5′-GTTTGCTGCATGACAGGCTAA AGACATC-3′
1500 Percival et al. (2008)
arsC AR-F
AR-R
5′-GTAATACGCTGGAGATGATCCG-3′
5′-TTTTCCTGCTTCATCAACGAC-3′
409 Sunita et al. (2012)
CS 5′CS
3′CS
5′-GGCATCCAAGCAGCAAG-3′
5′-AAGCAGACTTGACCTGA-3′
530 M73819
IntI IntI- F
IntI -R
5′-CTACCTCTCACTAGTGAGGGGCGG-3′
5′-GGGCAGCAGCGAAGTCGAGGC-3′
845 U12338
SulI SulI-F
SulI-R
5′-ATGGTGACGGTGTTCGGCAT-3′
5′CTAGGCATGATCTAACCCTC-3′
800 Galimand et al. (2003)
ISecp1 IS-F
IS-R
5′-TAAAAAACACAGGTGGAATTTAG-3′
5′-CCAGGAACCACGGAGC-3′
1000 This study
TN3 Tn3 P-1
Tn3 P-2
Tn3 P-3
5′-AACTGATCTTCCTGACCGTC- 3′
5′-TATGACCGATACGGCAGGTG- 3′
5′-TCAGCAATGAACGGACCAGC- 3′
752 Heffron et al. (1979)
TN21 Tn21 P-1
Tn21 P-2
Tn21 P-3
5′-AGAAAGTTCGTCCTGGGCTG- 3′
5′-TACTGCCGCGCATCAAGATC-3′
5′-GGCCAAGGACAAGAACCTGT-3′
1000 Kim et al. (2009), Brown et al. (1985)