Figure 3.

Dissection of the experimental nonadditivity factor (${\mathit{\gamma }}_{\mathit{Exp}}$) into transition-specific (${\mathit{\gamma }}_{12}$) and state-specific (${\mathit{\gamma }}_1$ and ${\mathit{\gamma }}_2$) contributions. Shown are the$\gamma$-values for the Rp/G cycle of Fig. 1c as applied to the HCN4 CNBD wt (bars with solid lines) and the frustration-free F689A mutant (bars with dotted lines). The gray region indicates $\gamma$-values that fall within the RT-benchmark window [e⁻¹, e], for which deviations from the additivity limit are assumed negligible. Error bars were obtained through error propagation.