Table 3.
Cellular visualization of EHV-1 DNA in trigeminal ganglia and sympathetic trunk ganglia using in situ hybridization.
| 30 dpi | 70 dpi | |||||||
|---|---|---|---|---|---|---|---|---|
| Viral localization (ISH) | Viral localization (ISH) | |||||||
| Tissue | Horse ID | Viral load gDNA gB | Neuron | Non-neuronal cells | Horse ID | Viral load gDNA gB | Neuron | Non-neuronal cells |
| TG | 1,651 | 5.41 × 103 | + | + | 905 | 4.19 × 101 | + | – |
| 1,621 | 2.58 × 104 | + | + | 900 | 9.93 × 101 | + | – | |
| 1,628 | 3.05 × 103 | + | + | 914 | 2.11 × 101 | + | – | |
| 908 | 3.51 × 101 | – | – | |||||
| ST | 1,651 | 1.98 × 105 | + | + | 905 | 3.82 × 104 | + | + |
| 1,638 | 1.80 × 104 | – | – | 914 | 1.57 × 104 | – | – | |
| 1,628 | 3.27 × 104 | + | – | 908 | 2.55 × 103 | – | – | |
| 1,629 | 5.07 × 103 | + | + | |||||
Viral load expressed in EHV-1 gDNA copies/106cells based on qPCR.
dpi, days post infection; ISH, in situ hybridization; TG, trigeminal ganglia; ST, sympathetic trunk ganglia; +, positive ISH signal; –, no ISH signal.