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. 2020 Sep 17;3:512. doi: 10.1038/s42003-020-01127-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Microsphere dilution produces values extremely close to the ground truth provided by calibrated flow cytometry, whereas the CFU protocol produces values more than an order of magnitude different, suggesting that CFU calibration greatly underestimates the number of cells in the sample. Bars show geometric mean and standard deviation. Team count per condition provided in Supplementary Data 3.