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. 2020 Sep 17;10:15244. doi: 10.1038/s41598-020-72179-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TAM-mediated induction of YFP reporter. (A) Representative scatter plots of the gating strategy to identify Cre activity by the expression of the YFP reporter among CD45+ cells on day 5 after initiation of treatment with 3 mg TAM PO for 5 days. (B) Testing of TAM dosage (2.4 mg and 1.2 mg) given IP and PO every other day for 5 days (n = 3–5/group). Data are presented as mean with standard error of the mean (SEM). Mixed model ANOVA test with repetitive measures was carried out to identify significant differences when testing different concentrations and delivery methods. *p ≤ 0.05.