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. 2020 Sep 17;10:15244. doi: 10.1038/s41598-020-72179-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Concentration of TAM measured in tissues. IP injection of TAM results in greater TAM concentration in tissues than PO administration. Comparison of TAM concentration between different tissues regardless the dose and delivery route of TAM (A; n = 21/group), and when given IP versus PO 3 mg and 6 mg dose from pooled tissues (B; n = 21/group). Comparison of TAM concentration given by IP (C) and PO (D) route (n = 4–5/group). Comparison of tissue TAM concentration between IP injection and PO when give TAM with high dose—6 mg (E) and low dose—3 mg (F) (n = 4–5/group). Data are presented as mean ± SEM. Mann–Whitney test was used for unpaired sample comparisons. *p ≤ 0.05, ***p ≤ 0.001, ****p ≤ 0.0001.