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. 2020 Sep 17;10:15244. doi: 10.1038/s41598-020-72179-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Highest induction of Cre in myeloid cells, and among T cells, in DP thymocytes. Frequency of YFP expressing cells (%) in (A) B cells (B220+ CD3−), (B) T cells (B220− CD3+), (C) CD4+ T cells (B220− CD3+ CD4+ CD8−), (D) CD8+ T cells (B220− CD3+ CD4− CD8+), (E) single positive CD4+ /CD8+, double positive (DP) and double negative (DN) thymocytes, (F) CD11b+ CD11c− macrophages, (G) CD11b− CD11c+, (H) CD11c+ MHCII+ and (I) CD11c+ CD11b+ dendritic cell populations, (J) CD11b+ Gr-1− and (K) CD11b+ Gr-1+ cells. Data was collected from 8 mice (CAG-Cre+) treated with TAM 3 mg PO. See Suppl. Information Fig. S2 for cell gating strategy, including BM B220 high and low cell gating. Data are presented as mean ± SEM. Mann–Whitney test was used for unpaired sample comparisons. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.