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. 2020 Sep 17;10:15286. doi: 10.1038/s41598-020-71857-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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GSC viability following Ccl2 silencing. (a) Ccl2 mRNA levels in GSCs transfected with 10 nM Ccl2 siRNA (siCcl2) or negative control siRNA (siCon) for 24 h (n = 6). (b) Representative western blot results for CCL2 (n = 4). (c) Representative western blot results for cyclin D1 from GSCs at 48-h post-transfection with siCcl2 or siCon (n = 4). (d) Representative images of GSCs and cell viability evaluated by WST-8 assay at 48-h post-transfection with siCcl2 or siCon (n = 4). Scale bar, 50 µm. (e) Schematic illustration of the antitumor effects of celecoxib in the mouse glioma model. Data represent the mean ± SD. *p < 0.05, **p < 0.01 vs. siCon by Student’s t test.