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. 2020 Sep 17;11(9):766. doi: 10.1038/s41419-020-02938-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a–c Dose- and time-dependent curves showing the percentage of inhibition of PC cells. BxPC-3, PANC-1 and SW1990 cells were treated with various concentrations of anlotinib (0–40 μM) for 24, 48 or 72 h. A CCK-8 assay was used to determine the viability of PC cells. d–e Colony formation was analysed. Each PC cell line was treated with 0, 5, 10 and 20 μM anlotinib for 24 h and cultured for 7 days. f–i An image (the length of the bar is 100 μm) of EdU staining of PC cells after anlotinib treatment for 24 h. The data are presented as the mean ± SD from three independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001).