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. 2020 Sep 17;10:15234. doi: 10.1038/s41598-020-71970-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The characteristics of human NSCs and neuroblastoma IMR-32-derived neuronal cells. (A) Schematic diagram of neuronal differentiation from human NSCs. (B) Bright-field image of human NSCs (i) and neurons (ii), the scale bar represents 50 μm. (C) Immunostaining images of neurons derived from human NSCs showing the expression of MAP2 (i), Tuj1 (β-tubulin III) (ii) and GAD67 (iii). The nuclei are counterstained with DAPI. The scale bar represents 20 μm. (D) Schematic diagram of neuronal differentiation from neuroblastoma IMR-32 cells. (E) Bright-field image of undifferentiated and differentiated IMR-32 cells. (F) Immunostaining images of neuronal cells derived from neuroblastoma IMR-32 cells showing the expression of MAP2 (i), Tuj1 (β-tubulin III) (ii) and GAD67 (iii). The nuclei were counterstained with DAPI. The scale bar represents 20 μm.