Figure 5.

Production of superoxide radicals by rat heart mitochondria-oxidizing palmitoyl-carnitine. Designations: 1. supporting substrate only; 2. palmitoyl-carnitine only, and 3. palmitoyl − carnitine + supporting substrate. Substrates: Figure 5(a) (pyruvate 2.5 mM + malate 2 mM), Figure 5(b) (glutamate 5 mM + malate 2 mM), and Figure 5(c). (succinate 5 mM). Experimental conditions are described in [93]. The incubation medium contained Amplex Red 2 μM, horse radish peroxidase 2 units, substrates as indicated above, and volume 1 ml. The reaction was initiated by addition of 50 μg of mitochondria. Initial rates were measured for 3 minutes. Numbers at the traces are the rates of H₂O₂ production in picomol H₂O₂/min/mg protein RHM. The rates were corrected for the time control rate with RHM incubated without added substrates. The figure was taken from [95].