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. 2020 Sep 17;11(9):770. doi: 10.1038/s41419-020-02974-0

Fig. 5. GP2 induces intestinal pro-glucagon expression through gut microbiota remodelling.

Fig. 5

a Schematic diagram of the experiment (n = 9–11). In brief, 6-week-old male mice were fed a high-fat diet for 10 weeks, and then the mice were randomly divided into four groups according to body weight and fasting blood glucose levels. Two groups of mice were treated with an antibiotic mix (ABX) for 1 week and then treated with or without GP2 combined with ABX for 3 weeks; the other two groups of mice were fed a high-fat diet for 1 week, and then treated with or without GP2 for 3 weeks. b Fasting blood glucose concentrations were measured after treatment with or without GP2 (200 mg/kg/d), and treatment with or without ABX for 3 weeks. The animals were fasted for 6 h before blood glucose determination. c The oral glucose tolerance test (OGTT) results of vehicle-, GP2-, vehicle+ ABX-and GP2+ABX-treated mice after glucose (2.0 g/kg) administration by gavage were plotted. d Area under the curve of glucose level in 90 min of OGTT in (c), e, f Quantitative mRNA expression of pro-glucagon in the ileum (e) and colon (f) after GP2 treatment combined with or without ABX treatment. The results are shown as the mean ± s.e.m., *P < 0.05, **P < 0.01, ***P < 0.001 compared with vehicle.