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. 2020 Aug 12;119(6):1157–1177. doi: 10.1016/j.bpj.2020.07.033

Figure 1.

Figure 1

From adherent to nonadherent 2D migration. Primary human effector T lymphocytes are shown. (A) Adhesive crawling on ICAM-1-treated substrate and (B) nonadherent migration on Pluronic F127-treated substrate. (i) Bright-field images. Blue lines correspond to the track of a particular cell for 12 min. Scale bars, 20 μm. See also Video S1. (ii) Representative tracks of motile cells in a single experiment (time lag = 20 s, duration 16 min, n > 100 cells). (iii) RICM images. Cell contact zone is dark for cells crawling on ICAM-treated surfaces, revealing an adhesion phenotype, and white for cells on nonadhesive surfaces, attesting the absence of adhesion to the surface (see also Fig. S1). Bars are standard deviations. ****p < 0.0001 (two-tailed Student’s t-test). Scale bars, 20 μm. (C) Image sequence of a cell migrating over adjacent stripes of adhesive ICAM-1 (red) and antiadhesive polyetheleneglycol (gray) prepared by LIMAP (72) with a width of 40 μm. The sequence is a merge of fluorescent images (ICAM-1, red), bright-field images (cell morphology, gray), and RICM images (adhesion zone, green). Scale bars, 20 μm. See also Video S3. To see this figure in color, go online.