Figure 8 – Pretreatment with fungal EVs reduced the mortality of G. mellonella larvae infected with C. albicans.

Survival curves of larvae pretreated with fungal EVs stored under different conditions (10 μl of a 10 μM suspension based on the sterol content) and then infected two days later with a lethal inoculum of C. albicans yeasts are shown. Statistical analysis was performed using oneway analysis of variance, and the survival differences between each EV groups and PBS were analyzed by individual paired Log-rank (Mantel-Cox) (Supplemental Table 1). Results are representative of two independent experiments.