Fig. 2.

Glycoproteomics analysis of the 341-VFNATR-346 glycopeptide derived from the RBD of the S1 spike SARS-CoV-2 glycoprotein containing the glycosylation site N343 found centered at 12.1 min in the LC elution profile. LC ES-MS (MS^e mode) containing the [M + 2H]²⁺ molecular ion found at m/z 1425.59 and corresponding to the 341-VFNATR-346 glycopeptide with a core-fucosylated bi-antennary N-glycan with fucosylated LacdiNAc antennae (permethylated N-glycan found at m/z 2674 on Figure 1B and Supplementary Table SI). The peaks in red correspond to glycopeptide fragment ions deriving from the [M + 2H]²⁺ molecular ion. “P” inside a circle corresponds to the peptide backbone of 341-VFNATR-346. Area corresponding to m/z 2120 to 2600 has a 5-fold magnification. The residues shown on top of a bracket have not had their antenna location unequivocally defined.