Fig. 2.

Confirmed hits increase sarcospan gene and protein expression in mdx myotubes. a Relative sarcospan gene expression in dystrophin-deficient H2K mdx cells treated with 0.5 to 50 μM of pharmacophore 1 or other compounds for 48 h. Gene expression was normalized to housekeeping gene β-Actin and vehicle-treated cells (0.2% DMSO). Data are represented as mean + SEM. n = 3–6. b–c Sarcospan immunoblot of dystrophin-deficient H2K mdx cells treated with 2.5 to 10 μM of compounds for 48 h. All cells were treated at day 2 of differentiation and harvested 48 h posttreatment. Sarcospan protein levels were quantified in ImageJ and normalized to GAPDH and vehicle-treated cells (0.2% DMSO). Cell lysates were probed between 2 and 4 times in independent western blots. Representative blots shown. Quantification shown below immunoblots includes all experiments. Data represents individual replicates and mean value. n = 3 per concentration. SSPN, sarcospan; R.U., relative units. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001