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. 2020 Sep 17;21:251. doi: 10.1186/s13059-020-02163-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Homeostasis of transposon-derived siRNAs. a Bar chart illustrating the relative enrichment of length-normalized 24-nt siRNAs derived from heterochromatic relative to euchromatic TEs across development, and in h1 mutant leaves. P values < 0.001 based on Student’s t test of heterochromatic vs euchromatic 24-nt siRNA enrichment differences between wild-type and h1 tissues are represented by ***. Various tissues and cell-types from different phases of the plant life-cycle are labeled and include those from published datasets [29, 39, 65–68]. b Relative enrichments of 24-nt siRNAs derived from heterochromatic relative to euchromatic TEs in RdDM and related mutants. Wild type (WT), drm1/2 cmt3, met1, and ros1 dml1/2 [69]; WT, cmt2, and suv4/5/6 [43]; WT, nrpd1a, nrpe1, shh1, and drm2 [21]; WT, clsy1, clsy2, clsy3, clsy4, clsy1/2, and clsy3/4 [19]. P values < 0.0001, < 0.001, and < 0.01 based on Student’s t test of heterochromatic vs euchromatic 24-nt siRNA enrichment differences between wild-type and mutants are represented by ****, ***, and **. c CLASSY1, CLASSY2, CLASSY3, and CLASSY4 transcript levels (TPM) during embryogenesis. d Metaplots of 24-nt siRNA levels overlapping long heterochromatic TEs in 6 DAP embryo (red; emb), endosperm (blue; endo), or seed coat (yellow; sc) tissues based on [65]. See also Additional file 2: Figure S6