Fig. 6.

The protein P5 binding to the BECN1 ring-like structure regulates RABV replication via the BECN1-mediated signaling pathway. a, b N2a cells were transfected with Flag-P5 (a) or cotransfected with Flag-P5 and two siRNAs targeting Becn1 (b) for 48 h, and then harvested for western blotting analysis with the indicated antibodies. c N2a cells were cotransfected with Flag-P5 and the shRNA/siRNA targeting Akt or Mtor or Ampk or Mapk for 12 h, and infected with RABV HEP-Flury strain at an MOI = 2 for 36 h. Cells were harvested for western blotting analysis with the indicated antibodies. d Proposed model showing how P5 protein regulates RABV replication. Data are representative of 3 independent experiments