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. 2020 Sep 18;16:345. doi: 10.1186/s12917-020-02568-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Anti-PCV2 activities of Paeonol, Cepharanthine or Curcumin detected by qPCR and Western blot. The anti-PCV2 effect of compounds was evaluated by qPCR and Western blot. Cells were incubated with 10^4.4 TCID₅₀ of PCV2 for 2 h, then tested compounds were added respectively and cultured for 48 h. Cells were collected and relative assay perform. a The expression of the Cap gene was detected by qPCR; b The expression of the Cap protein was detected by Western blot. GraphPad Prism 5.0 was used for the statistical analysis. All data were presented in Mean ± SEM. Different letters to indicate significant differences between groups (p<0.05)